MightyMountTM Antifade Fluorescence Mounting Medium (aqueous)
(HB9854)
Certificate of Analysis
Product overview
| Name | MightyMountTM Antifade Fluorescence Mounting Medium (aqueous) |
| Biological description |
Overview MightyMountTM Antifade Fluorescence Mounting Medium (aqueous) is an ideal formulation for prevention of photobleaching of fluorescent proteins and dyes during fluorescent imaging. It is easy to use with an ideal refractive index and provides effective prevention of photobleaching. Applications: IHC(IF), ICC, Cellular imaging, Super-resolution microscopy
We supply a full range of mounting media for a range of experimental needs: Hardset:
Aqueous: |
| Description |
Antifade aqueous fluorescence mounting medium for use in IHC(IF) and ICC. |
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Images
Figure 1. βIII-tubulin and Neurofilament light staining in rat cerebellum. Mounted using MightyMountTM Antifade Fluorescence Mounting Medium (aqueous).
4% PFA fixed 40µm horizontal rat brain sections were stained for βIII-tubulin (HB6639) and Neurofilament light (HB7266) with DAPI (HB0747) used as a nuclear counterstain. Sections were mounted using MightyMountTM Antifade Fluorescence Mounting Medium (aqueous). For a full protocol and more information on IHC(IF) please see our IHC(IF) protocol.
Figure 2. GFAP and NeuN staining in rat hippocampus. Mounted using MightyMountTM Antifade Fluorescence Mounting Medium (aqueous).
4% PFA fixed 40µm horizontal rat brain sections were stained for GFAP (HB8267) and NeuN (HB6498) with DAPI (HB0747) used as a nuclear counterstain. Sections were mounted using MightyMountTM Antifade Fluorescence Mounting Medium (aqueous). For a full protocol and more information on IHC(IF) please see our IHC(IF) protocol.
Figure 3. MBP and Neurofilament L staining in rat cerebellum. Mounted using MightyMountTM Antifade Fluorescence Mounting Medium (aqueous).
4% PFA fixed 40µm horizontal rat brain sections were stained for MBP (HB8014) and Neurofilament L (HB7266) with DAPI (HB0747) used as a nuclear counterstain. Sections were mounted using MightyMountTM Antifade Fluorescence Mounting Medium (aqueous). For a full protocol and more information on IHC(IF) please see our IHC(IF) protocol.
Figure 4. Antifade performance of MightyMountTM Antifade Fluorescence Mounting Medium (aqueous) compared to VECTASHIELD® and Fluoromount-G™.
Hello Bio MightyMountTM Antifade Fluorescence Mounting Medium (aqueous) shows strong antifade characteristics against a range of fluorophores and compares favorably against VECTASHIELD® and Fluoromount-G™. Method: HEK293T cells were cultured on coverslips then stained with DAPI (HB0747) and with a mouse anti-β-tubulin antibody (HB6491) with Alexa Fluor 488 or DyLight 550 conjugated secondary antibodies. Following mounting with either MightyMountTM Antifade Fluorescence Mounting Medium (aqueous), VECTASHIELD® or Fluoromount-G™, slides were imaged on a confocal microscope. Imaging was carried out as a timeseries at 100% laser power with the normalized intensity being calculated compared to the first exposure.
Figure 5. GluN1 and NeuN staining in rat cerebellum. Mounted using MightyMountTM Antifade Fluorescence Mounting Medium (aqueous).
Glyoxal fixed (9% glyoxal, 8% acetic acid, pH4) 40µm horizontal rat brain sections were stained for GluN1 (HB7535) and NeuN (HB6498) with DAPI (HB0747) used as a nuclear counterstain. Sections were mounted using MightyMountTM Antifade Fluorescence Mounting Medium (aqueous). For a full protocol and more information on IHC(IF) please see our IHC(IF) protocol.
Figure 6. βIII-tubulin and Neurofilament light staining in rat cortex. Mounted using MightyMountTM Antifade Fluorescence Mounting Medium (aqueous).
4% PFA fixed 40µm horizontal rat brain sections were stained for βIII-tubulin (HB6639) and Neurofilament light (HB7266) with DAPI (HB0747) used as a nuclear counterstain. Sections were mounted using MightyMountTM Antifade Fluorescence Mounting Medium (aqueous). For a full protocol and more information on IHC(IF) please see our IHC(IF) protocol.
Figure 7. βIII-tubulin and Neurofilament light staining in rat cerebellum. Mounted using MightyMountTM Antifade Fluorescence Mounting Medium (aqueous).
4% PFA fixed 40µm horizontal rat brain sections were stained for βIII-tubulin (HB6639) and Neurofilament light (HB7266) with DAPI (HB0747) used as a nuclear counterstain. Sections were mounted using MightyMountTM Antifade Fluorescence Mounting Medium (aqueous). For a full protocol and more information on IHC(IF) please see our IHC(IF) protocol.
Figure 8. βIII-tubulin and Neurofilament light staining in rat cerebellum. Mounted using MightyMountTM Antifade Fluorescence Mounting Medium (aqueous).
4% PFA fixed 40µm horizontal rat brain sections were stained for βIII-tubulin (HB6639) and Neurofilament light (HB7266) with DAPI (HB0747) used as a nuclear counterstain. Sections were mounted using MightyMountTM Antifade Fluorescence Mounting Medium (aqueous). For a full protocol and more information on IHC(IF) please see our IHC(IF) protocol.
Figure 9. MBP and Neurofilament L staining in rat dentate gyrus. Mounted using MightyMountTM Antifade Fluorescence Mounting Medium (aqueous).
4% PFA fixed 40µm horizontal rat brain sections were stained for MBP (HB8014) and Neurofilament L (HB7266) with DAPI (HB0747) used as a nuclear counterstain. Sections were mounted using MightyMountTM Antifade Fluorescence Mounting Medium (aqueous). For a full protocol and more information on IHC(IF) please see our IHC(IF) protocol.
Figure 10. Neurofilament L staining in rat dentate gyrus. Mounted using MightyMountTM Antifade Fluorescence Mounting Medium (aqueous).
4% PFA fixed 40µm horizontal rat brain sections were stained for Neurofilament L (HB7266) with DAPI (HB0747) used as a nuclear counterstain. Sections were mounted using MightyMountTM Antifade Fluorescence Mounting Medium (aqueous). For a full protocol and more information on IHC(IF) please see our IHC(IF) protocol.
Figure 11. MBP and Neurofilament L staining in rat cerebellum. Mounted using MightyMountTM Antifade Fluorescence Mounting Medium (aqueous).
4% PFA fixed 40µm horizontal rat brain sections were stained for MBP (HB8014) and Neurofilament L (HB7266) with DAPI (HB0747) used as a nuclear counterstain. Sections were mounted using MightyMountTM Antifade Fluorescence Mounting Medium (aqueous). For a full protocol and more information on IHC(IF) please see our IHC(IF) protocol.
Figure 12. MBP and Neurofilament L staining in rat subiculum. Mounted using MightyMountTM Antifade Fluorescence Mounting Medium (aqueous).
4% PFA fixed 40µm horizontal rat brain sections were stained for MBP (HB8014) and Neurofilament L (HB7266) with DAPI (HB0747) used as a nuclear counterstain. Sections were mounted using MightyMountTM Antifade Fluorescence Mounting Medium (aqueous). For a full protocol and more information on IHC(IF) please see our IHC(IF) protocol.
Figure 1. βIII-tubulin and Neurofilament light staining in rat cerebellum. Mounted using MightyMountTM Antifade Fluorescence Mounting Medium (aqueous).
4% PFA fixed 40µm horizontal rat brain sections were stained for βIII-tubulin (HB6639) and Neurofilament light (HB7266) with DAPI (HB0747) used as a nuclear counterstain. Sections were mounted using MightyMountTM Antifade Fluorescence Mounting Medium (aqueous). For a full protocol and more information on IHC(IF) please see our IHC(IF) protocol.
Figure 2. GFAP and NeuN staining in rat hippocampus. Mounted using MightyMountTM Antifade Fluorescence Mounting Medium (aqueous).
4% PFA fixed 40µm horizontal rat brain sections were stained for GFAP (HB8267) and NeuN (HB6498) with DAPI (HB0747) used as a nuclear counterstain. Sections were mounted using MightyMountTM Antifade Fluorescence Mounting Medium (aqueous). For a full protocol and more information on IHC(IF) please see our IHC(IF) protocol.
Figure 3. MBP and Neurofilament L staining in rat cerebellum. Mounted using MightyMountTM Antifade Fluorescence Mounting Medium (aqueous).
4% PFA fixed 40µm horizontal rat brain sections were stained for MBP (HB8014) and Neurofilament L (HB7266) with DAPI (HB0747) used as a nuclear counterstain. Sections were mounted using MightyMountTM Antifade Fluorescence Mounting Medium (aqueous). For a full protocol and more information on IHC(IF) please see our IHC(IF) protocol.
Figure 4. Antifade performance of MightyMountTM Antifade Fluorescence Mounting Medium (aqueous) compared to VECTASHIELD® and Fluoromount-G™.
Hello Bio MightyMountTM Antifade Fluorescence Mounting Medium (aqueous) shows strong antifade characteristics against a range of fluorophores and compares favorably against VECTASHIELD® and Fluoromount-G™. Method: HEK293T cells were cultured on coverslips then stained with DAPI (HB0747) and with a mouse anti-β-tubulin antibody (HB6491) with Alexa Fluor 488 or DyLight 550 conjugated secondary antibodies. Following mounting with either MightyMountTM Antifade Fluorescence Mounting Medium (aqueous), VECTASHIELD® or Fluoromount-G™, slides were imaged on a confocal microscope. Imaging was carried out as a timeseries at 100% laser power with the normalized intensity being calculated compared to the first exposure.
Figure 5. GluN1 and NeuN staining in rat cerebellum. Mounted using MightyMountTM Antifade Fluorescence Mounting Medium (aqueous).
Glyoxal fixed (9% glyoxal, 8% acetic acid, pH4) 40µm horizontal rat brain sections were stained for GluN1 (HB7535) and NeuN (HB6498) with DAPI (HB0747) used as a nuclear counterstain. Sections were mounted using MightyMountTM Antifade Fluorescence Mounting Medium (aqueous). For a full protocol and more information on IHC(IF) please see our IHC(IF) protocol.
Figure 6. βIII-tubulin and Neurofilament light staining in rat cortex. Mounted using MightyMountTM Antifade Fluorescence Mounting Medium (aqueous).
4% PFA fixed 40µm horizontal rat brain sections were stained for βIII-tubulin (HB6639) and Neurofilament light (HB7266) with DAPI (HB0747) used as a nuclear counterstain. Sections were mounted using MightyMountTM Antifade Fluorescence Mounting Medium (aqueous). For a full protocol and more information on IHC(IF) please see our IHC(IF) protocol.
Figure 7. βIII-tubulin and Neurofilament light staining in rat cerebellum. Mounted using MightyMountTM Antifade Fluorescence Mounting Medium (aqueous).
4% PFA fixed 40µm horizontal rat brain sections were stained for βIII-tubulin (HB6639) and Neurofilament light (HB7266) with DAPI (HB0747) used as a nuclear counterstain. Sections were mounted using MightyMountTM Antifade Fluorescence Mounting Medium (aqueous). For a full protocol and more information on IHC(IF) please see our IHC(IF) protocol.
Figure 8. βIII-tubulin and Neurofilament light staining in rat cerebellum. Mounted using MightyMountTM Antifade Fluorescence Mounting Medium (aqueous).
4% PFA fixed 40µm horizontal rat brain sections were stained for βIII-tubulin (HB6639) and Neurofilament light (HB7266) with DAPI (HB0747) used as a nuclear counterstain. Sections were mounted using MightyMountTM Antifade Fluorescence Mounting Medium (aqueous). For a full protocol and more information on IHC(IF) please see our IHC(IF) protocol.
Figure 9. MBP and Neurofilament L staining in rat dentate gyrus. Mounted using MightyMountTM Antifade Fluorescence Mounting Medium (aqueous).
4% PFA fixed 40µm horizontal rat brain sections were stained for MBP (HB8014) and Neurofilament L (HB7266) with DAPI (HB0747) used as a nuclear counterstain. Sections were mounted using MightyMountTM Antifade Fluorescence Mounting Medium (aqueous). For a full protocol and more information on IHC(IF) please see our IHC(IF) protocol.
Figure 10. Neurofilament L staining in rat dentate gyrus. Mounted using MightyMountTM Antifade Fluorescence Mounting Medium (aqueous).
4% PFA fixed 40µm horizontal rat brain sections were stained for Neurofilament L (HB7266) with DAPI (HB0747) used as a nuclear counterstain. Sections were mounted using MightyMountTM Antifade Fluorescence Mounting Medium (aqueous). For a full protocol and more information on IHC(IF) please see our IHC(IF) protocol.
Figure 11. MBP and Neurofilament L staining in rat cerebellum. Mounted using MightyMountTM Antifade Fluorescence Mounting Medium (aqueous).
4% PFA fixed 40µm horizontal rat brain sections were stained for MBP (HB8014) and Neurofilament L (HB7266) with DAPI (HB0747) used as a nuclear counterstain. Sections were mounted using MightyMountTM Antifade Fluorescence Mounting Medium (aqueous). For a full protocol and more information on IHC(IF) please see our IHC(IF) protocol.
Figure 12. MBP and Neurofilament L staining in rat subiculum. Mounted using MightyMountTM Antifade Fluorescence Mounting Medium (aqueous).
4% PFA fixed 40µm horizontal rat brain sections were stained for MBP (HB8014) and Neurofilament L (HB7266) with DAPI (HB0747) used as a nuclear counterstain. Sections were mounted using MightyMountTM Antifade Fluorescence Mounting Medium (aqueous). For a full protocol and more information on IHC(IF) please see our IHC(IF) protocol.
Biological Data
| Application notes |
Protocol for use of mounting media IHC(IF)
For more information on IHC(IF) including tips on how to mount sections, please see our IHC(IF) protocol
ICC
For more information on ICC please see our ICC protocol |
Solubility & Handling
| Storage instructions |
+4°C or -20°C long-term. Protect from light. |
| Important | This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use. |
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